First report of Ophiostoma gilletteae (Ascomycota, Ophiostomatales) associated with Dendroctonus valens (Coleoptera: Scolytinae) on Pinus montezumae in the State of Mexico
Keywords:
Ocote, ophiostomatoid fungi, molecular characterization, PCR, PinusAbstract
Pinus montezumae D.Don is a host species of the bark beetle Dendroctonus valens LeConte (Coleoptera: Scolytinae), whose colonization is associated with symbiotic fungi (Ophiostomatales) that help interfere with the tree's defense mechanisms. The objective of this study was to identify and characterize, morphologically and molecularly, the ophiostomatoid fungus associated with D. valens in a P. montezumae stand in the Monte Comunal Isidro Fabela area, Jocotitlan, State of Mexico, Mexico. Following the collection of D. valens specimens in 2024, Ophiostoma gilletteae Marinc., Z.W. de Beer & M.J. Wingf. (Ascomycota, Ophiostomatales) was isolated and identified. This represents the first record of this fungus associated with P. montezumae in Mexico, and expands the knowledge of the fungal diversity associated with this bark beetle.
References
Armendáriz-Toledano, F., Zúñiga, G., García-Román, L. J., Valerio-Mendoza, O., & García-Navarrete, P. G. (2018). Guía ilustrada para identificar a las especies del género Dendroctonus presentes en México y Centroamérica. Instituto Politécnico Nacional.
Barnett, H. L., & Hunter, B. B. (1998). Illustrated Genera of Imperfect Fungi. The American Phytopathological Society Press.
Cibrián Tovar, D., Méndez Montiel, J. T., Campos Bolaños, R., Yates III, H. O., & Flores Lara, J. E. (1995). Insectos forestales de México/Forest insects of Mexico. Universidad Autónoma Chapingo.
Glass, N. L., & Donaldson, G. C. (1995). Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology, 61(4), 1323-1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
Hall, T. A. (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95-98.
Kumar, S., Stecher, G., Suleski, M., Sanderford, M., Sharma, S., & Tamura, K. (2024). MEGA12: Molecular evolutionary genetic analysis version 12 for adaptive and green computing. Molecular Biology and Evolution, 41, msae263. https://doi.org/10.1093/molbev/msae263
Marincowitz, S., Duong, T. A., Taerum, S. J., de Beer, Z. W., & Wingfield, M. J. (2020). Fungal associates of an invasive pine-infesting bark beetle, Dendroctonus valens, including seven new Ophiostomatalean fungi. Persoonia, 45, 177-195. https://doi.org/10.3767/persoonia.2020.45.07
Marmolejo, J. G., & Butin, H. (1990). New conifer-inhabiting species of Ophiostoma and Ceratocystiopsis (Ascomycetes, Microascales) from Mexico. Sydowia, 42, 193-199.
Martínez-González, C. R., Ramírez-Mendoza, R., Jiménez-Ramírez, J., Gallegos-Vázquez, C., & Luna-Vega, I. (2017). Improved method for genomic DNA extraction for Opuntia Mill. (Cactaceae). Plant Methods, 13, 82. https://doi.org/10.1186/s13007-017-0234-y
Paine, T. D., Raffa, K. F., & Harrington, T. C. (1997). Interactions among scolytid bark beetles, their associated fungi, and live host conifers. Annual Review of Entomology, 42, 179-206. https://doi.org/10.1146/annurev.ento.42.1.179
Six, D. L., & Wingfield, M. J. (2011). The role of phytopathogenicity in bark beetle-fungus symbioses: A challenge to the classic paradigm. Annual Review of Entomology, 56, 255-272. https://doi.org/10.1146/annurev-ento-120709-144839
Terrazas Sánchez, G. (2006). Ecología química y hongos asociados de Dendroctonus valens Le Conte (Coleoptera: Scolytidae) [Tesis de maestría inédita]. Universidad Autónoma Chapingo.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. En M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315-322). Academic Press. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Yan, Z., Sun, J., Don, O., & Zhang, Z. (2005). The red turpentine beetle, Dendroctonus valens LeConte (Scolytidae): An exotic invasive pest of pine in China. Biodiversity and Conservation, 14, 1735-1760. https://doi.org/10.1007/s10531-004-0697-9
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Omar Alejandro Pérez-Vera, José Francisco Reséndiz-Martínez, David Cibrián-Tovar, Erick Velázquez-Reyes
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
