In vitro GROWTH INHIBITION OF Fusarium oxysporum SCHLTDL., WITH Argemone ochroleuca SWEET (PAPAVERACEAE).
Keywords:
strawberry disease, vegetal extract, chicaloteAbstract
The fungus Fusarium oxysporum Schltdl. (Ascomycetes) causes strawberry diseases. Synthetic
fungicides are primarily used for their control; however, these fungicides have a negative impacts on the environment. An alternative control strategy is the use of antifungal compounds of botanical origin. The objective of this study was to evaluate extracts of Argemone ochroleuca
Sweet (Papaveraceae) on F. oxysporum viability under in vitro conditions. Strawberry plants with characteristic foliar disease symptoms were collected, the isolated fungi were purified and characterized both morphologically and molecularly. Growth inhibition was evaluated
using three concentrations of the extract (13, 23, and 31%). The isolates were identified as F. oxysporum. Their growth inhibition was by 66% on the ninth day using 13% of the extract,
whereas the 23 and 31% concentrations inhibited the growth of F. oxysporum entirely on the
third day of evaluation.
References
Ajwa HA, Klose S, Nelson SD, Minuto A, Gullino ML, Lamberti F, Lopez-Aranda JM. 2003. Alternatives to methyl bromide in strawberry production in the United States of America and the Mediterranean region. Phytopathologia Mediterranea 42(3): 220-244.
Bárcenas-Santana D, Guillén-Sánchez D, Yazmín-Basaldua C, Ramos-García ML, Valle-de la Paz M. 2019. Etiología de la secadera de la fresa (Fragaria spp.) en Morelos, México. Revista Mexicana de Fitopatología 37(3): 1-10. https://doi.org/10.18781/R.MEX.FIT.1904-5
Brahmachari G, Gorai D, Roy R. 2013. Argemone mexicana: Chemical and pharmacological aspects. Revista Brasileira de Farmacognosia 23(3): 559-575. https://doi.org/10.1590/S0102-695X2013005000021
Calderón RG. 1991. Familia Papaveraceae. In: Rzedowski J, editor. Flora del Bajío y de regiones adyacentes. Fascículo 54. Pátzcuaro, Instituto de Ecología, A.C., Centro Regional del Bajío. P. 185-188.
Castro FJ, Dávalos G. 1990. Etiología de “la secadera” o pudrición de la raíz y corona de la fresa en Irapuato, Guanajuato. Revista Mexicana de Fitopatología 8: 80-86. https://doi.org/10.18781/r.mex.fit.1806-1
Ceja-Torres LF, Mora-Aguilera G, Téliz D, Mora-Aguilera A, Sánchez-García P, Muñoz-Ruíz C, La Torre-Almaraz C. 2008. Ocurrencia de hongos y etiología de la secadera de la fresa con diferentes sistemas de manejo agronómico. Agrociencia 42(4): 451-461.
Chidichima LDS, Nozaki MDH, Hendges C, Gaias WL. 2018. Growth and etiology strawberry anthracnosis causal. Acta Iguazu 7(4): 24-34.
[COFEPRIS] Comisión Federal para la Protección contra Riesgos Sanitarios. [internet]. 2020. Consulta de Registros Sanitarios de Plaguicidas, Nutrientes Vegetales y LMR. [cited 2020 May 01]. Disponible en: http://siipris03.cofepris.gob.mx/Resoluciones/Consultas/ConWebRegPlaguicida.asp.
Dellaporta SL, Wood J, Hicks JB. 1983. A plant DNA minipreparation: Version II. Plant Molecular
Biology Reporter 1(4): 19-21. https://doi.org/10.1007/BF02712670
De los Santos B, Barrau C, Romero F. 2003. Strawberry fungal diseases. Journal of Food Agriculture and Environment 1(3): 129-132. De Rodríguez DJ, Hernández-Castillo D, Angulo-Sánchez JL, Rodríguez-García R, Villarreal Quintanilla JA, Lira-Saldivar RH. 2007. Antifungal activity in vitro of Flourensia spp. extracts on Alternaria sp., Rhizoctonia solani, and Fusarium oxysporum. Industrial Crops and Products 25(2): 111-116. https://doi.org/10.1016/j.
indcrop.2006.08.007
[FAOSTAT] Statistical databases. Food and Agriculture Organization of the United Nations. [internet]. 2018. [cited 2019 Oct 19]. Disponible en: http://www.fao.org/faostat/es/?#data/QC.
Gahukar RT. 2016. Plant-derived products in crop protection: Effects of various application methods on pests and diseases. Phytoparasitica 44(3): 379-391. https://doi.org/10.1007/s12600-016-0524-3
Gardes M, Bruns TD. 1993. ITS primers with enhanced specificity for basidiomicetes application to the identification of mycorrhizae and rusts. Molecular Ecology 2(2): 113-118. https://doi.org/10.1111/j.1365-294x.1993.tb00005.x
Gerwick BC, Sparks TC. 2014. Natural products for pest control: An analysis of their role, value and future. Pest Management Science 70(8): 1169-1185. https://doi.org/10.1002/ps.3744
Hernández-Ruíz J, Bernal J, Gonzales-Castañeda J, Ruiz-Nieto JE, Mireles-Arriaga AI. 2020. Argemone ochroleuca: (Papaveraceae), alkaloid potential source for agricultural and medicinal uses. Tropical and subtropical agroecosystems 23 (31): In press.
Isman MB, Seffrin R. 2014. Natural insecticides from the Annonaceae: A unique example for developing biopesticides. In: Singh D, editor. Advances in Plant Biopesticides. New Delhi, Springer. P. 21-33.
León-López L, Guzmán-Ortíz DLA, García Berumen JA, Chávez Marmolejo CG, Peña-Cabriales JJ. 2014. Consideraciones para mejorar la competitividad de la región "El Bajío" en la producción nacional de fresa. Revista Mexicana de Ciencias Agrícolas 5(4): 673-686.
López-Medina J, López-Aranda JM, Medina-Míngez JJ, Miranda-Enamorado L, Soria-Navarro C, Domínguez-Romero FJ, Vázquez-Ortiz E, Flores-Gil F. 2007. Strawberry production from transplants fumigated with methyl bromide alternatives. Spanish Journal of Agricultural Research (3): 407-416.
Mariscal-Amaro LA, Rivera-Yerena A, Dávalos-González PA, Ávila-Martínez D. 2017. Situación actual de hongos asociados a la secadera de la fresa (Fragaria×ananassa duch.) en Guanajuato, México. Agrociencia 51(6): 673-681.
Juárez-García et al. Martínez AM, Aguado-Pedraza AJ, Viñuela E, Rodríguez-Enríquez CL, Lobit P, Gómez B, Pineda S. 2017. Effects of ethanolic extracts of Argemone ochroleuca (Papaveraceae) on the food consumption and development of Spodoptera frugiperda (Lepidoptera: Noctuidae). Florida Entomologist 100(2): 339-345. https://doi.org/10.1653/024.100.0232
Mdee LK, Masoko P, Eloff JN. 2009. The activity of extracts of seven common invasive plant species on fungal phytopathogens. South African Journal of Botany 75(2): 375-379. https://doi.org/10.1016/j.sajb.2009.02.003
Nelson PE, Toussoun TA, Marasas WFO. 1983. Fusarium Species: An Illustrated Manual for Identification. Penn State University Press. University Park, USA.
Ochoa YM, Cerna E, Landeros J, Hernández S, Delgado JC. 2012. Evaluación in vitro de la actividad antifúngica de cuatro extractos vegetales metanólicos para el control de tres especies de Fusarium spp. Phyton 81(1): 69-73.
Pedraza RO, Motok J, Salazar SM, Ragout A, Mentel MI, Tortora ML, Guerrero-Molina MF, Wini BB, Díaz-Ricci JC. 2010. Growth-promotion of strawberry plants inoculated with Azospirillum brasilense. World Journal of Microbiology and Biotechnology 26(2): 265-272. https://doi.org/10.1007/s11274-009-0169-1
Rizvi R, Mahmood I, Tiyagi SA, Khan Z. 2012. Effect of some botanicals for the management of plant-parasitic nematodes and soil-inhabiting fungi infesting chickpea. Turkish Journal of Agriculture and Forestry 36(6): 710-719. https://doi.org/10.3906/sag-1208-12
Roberts MF, Wink M. 1998. Alkaloids: Biochemistry, Ecology, and Medicinal Applications. Springer Science Business Media. New York, USA.
Rzedowski C, Rzedowski G. 2001. Flora fanerogámica del Valle de México. Instituto de Ecología/Comisión Nacional para el Conocimiento y Uso de la Biodiversidad. Michoacán, México.
[SAS] Statistical Analysis Systems. [internet]. 2002. SAS/STAT Users guide, version 9. SAS Institute Inc. North Carolina, USA.
Sharma A, del Carmen Flores-Vallejo R, Cardoso-Taketa A, Villarreal ML. 2017. Antibacterial activities of medicinal plants used in Mexican traditional medicine. Journal of ethnopharmacology 208: 264-329. https://doi.org/10.1016/j.jep.2016.04.045
[SIAP] Servicio de Información Agroalimentaria y Pesquera. [internet]. 2018. Resumen nacional por estado. [cited 2019 Nov 01]. Disponible en: http://infosiap.siap.gob.mx:8080/agricola_siap_gobmx/ResumenProducto.do.
Siddiqui IA, Shaukat SS, Khan GH, Zaki MJ. 2002. Evaluation of Argemone mexicana for control of root?infecting fungi in tomato. Journal of Phytopathology 150(6): 321-329. https://doi.org/10.1046/j.1439-0434.2002.00762.x
Singh A, Singh S, Singh S, Singh T, Singh V, Pandey V, Singh U. 2009. Fungal spore germination inhibition by alkaloids dehydrocorydalmine and oxyberberine. Journal of Plant Protection Research 49(3): 287-289. https://doi.org/10.2478/v10045-009-0046-9
Singh S, Singh A, Keshariwala M, Singh TD, Singh VP, Pandey VB, Singh UP. 2010. The mixture of tertiary and quaternary alkaloids isolated from Argemone ochroleuca inhibits spore germination of some fungi. Archives of Phytopathology and Plant Protection 43(13): 1249-
https://doi.org/10.1080/03235400802404627
Tortora ML, Díaz-Ricci JC, Pedraza RO. 2012. Protection of strawberry plants (Fragaria ananassa Duch.) against anthracnose disease induced by Azospirillum brasilense. Plant and Soil 356(1): 279-290. https://doi.org/10.1007/s11104-011-0916-6
Villa-Martínez A, Pérez-Leal R, Morales-Morales HA, Basurto-Sotelo M, Soto-Parra JM, Martínez-Escudero E. 2015. Situación actual en el control de Fusarium spp. y evaluación de la actividad antifúngica de extractos vegetales. Acta Agronómica 64(2): 194-205. https://doi.
org/10.15446/acag.v64n2.43358
White TJ, Burns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocol: A Guide to Methods and Applications. San Diego, Academic Press. P.
-322.
Zhang D, Yan D, Fang W, Huang B, Wang X, Wang X, Wang Q. 2019. Chloropicrin alternated with biofumigation increases crop yield and modifies soil bacterial and fungal communities in strawberry production. Science of the Total Environment 675: 615-622. https://doi.org/10.1016/j.scitotenv.2019.04.222.
