PROPOSAL FOR A CULTURE MEDIUM FOR THE IN VITRO ROOTING OF AGAVE ANGUSTIFOLIA HAW (ASPARAGALES: ASPARAGACEAE)
IN VITRO ROOTING OF AGAVE
Keywords:
acclimatization, 1-naphthaleneacetic acid, auxinsAbstract
The objective of the work was to determine the concentration and type of auxin for the rooting of Agave angustifolia Haw, and ex vitro acclimatization methodology. 24 plants were established in a liquid medium; the auxins used were: 1-naphthaleneacetic acid (ANA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA) in concentrations of 0, 0.3, 0.6 and 0.9 mg L-1, one week later 12 plants were placed in semi-solid medium without auxins and the rest were kept in liquid medium. The variables evaluated were: rooting percentage; number (NR), and length of roots (LR). The best results were obtained with ANA to 67 – 100% rooting. The NR and LR were higher in semi-solid medium with a range of 1.87 - 13.75 and for liquid medium with 0.27 - 0.61 cm, respectively. With the acclimatization methodology, survival was greater than 90%. It was determined that with ANA the best results are obtained for NR and LR.
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