In vitro efficacy of plant extracts against Fusarium species isolated from forage maize grains used for livestock feed
Keywords:
Feed corn, fungi, extractsAbstract
Due to the health risk posed by Fusarium contamination in forage maize (Zea mays L.) intended for silage, the objective of this study was to evaluate in vitro the inhibitory effect of mustard, governor, cinnamon, and citrus extracts on strains of F. oxysporum, F. verticillioides, and F. chlamydosporum isolated from maize grains. The results showed that mustard extract achieved mycelial control greater than 91 % and spore inhibition of 85 % (250 ppm), while governor extract exhibited 99.99 % efficacy against F. chlamydosporum. Although cinnamon and citrus extracts reached control levels close to 90 %, they required very high doses (30,000 ppm). The biological effectiveness is attributed to compounds such as cinnamaldehyde and flavonoids, which alter the cellular structure of the fungus. These extracts represent a sustainable natural alternative compared with chemical fungicides, allowing the mitigation of economic losses and reducing the risk of mycotoxin contamination in livestock feed.
References
Bamidele, M. O., Álvarez Pérez, O. B., Sandoval-Cortes, J., Flores-López, M. L., Chavez-González, M. L., & Aguilar, C. N. (2024). Polyphenolic bioactive compounds from Larrea tridentata (DC.) Coville: Extraction, characterization, antioxidant, and antifungal activities. Research Square. https://doi.org/10.21203/rs.3.rs-4370220/v1
Bautista Zúñiga, F., Delfín González, H., Palacio Prieto, J. L., & Delgado Carranza, M. del C. (2004). Técnicas de muestreo para manejadores de recursos naturales. Universidad Nacional Autónoma de México; Universidad Autónoma de Yucatán; Consejo Nacional de Ciencia y Tecnología; Instituto Nacional de Ecología.
Barnett, H. L., & Hunter, B. B. (1998). Illustrated genera of imperfect fungi. aps Press.
Carmello, C. R., Magri, M. M. R., & Cardoso, J. C. (2022). Cinnamon extract and sodium hypochlorite in the in vitro control of Fusarium oxysporum f. sp. lycopersici and Alternaria alternata from tomato. Journal of Phytopathology, 170(11-12), 802-810. https://doi.org/10.1111/jph.13143
Castro, J. C., Pante, G. C., Centenaro, B. M., De Almeida, R. T. R., Pilau, E. J., Dias Filho, B. P., Mossini, S. A. G., De Abreu Filho, B. A., Matioli, G., & Machinski Junior, M. (2020). Antifungal and antimycotoxigenic effects of Zingiber officinale, Cinnamomum zeylanicum and Cymbopogon martinii essential oils against Fusarium verticillioides. Food Additives & Contaminants: Part A, 37(9), 1531-1541. https://doi.org/10.1080/19440049.2020.1778183
Cerna-Chávez, E., Malacara-Herrera, I. del R., Ochoa-Fuentes, Y. M., & Hernández-Juárez, A. (2023). Evaluación in vitro de extractos vegetales adicionados con nanopartículas para el control de Fusarium oxysporum. Ecosistemas y Recursos Agropecuarios, 10(2), e3424. https://doi.org/10.19136/era.a10n2.3424
Dinolfo, M. I., Martínez, M., Castañares, E., & Arata, A. F. (2022). Fusarium in maize during harvest and storage: a review of species involved, mycotoxins, and management strategies to reduce contamination. European Journal of Plant Pathology, 164, 151-166. https://doi.org/10.1007/s10658-022-02548-0
Drakopoulos, D., Luz, C., Torrijos, R., Meca, G., Weber, P., Bänziger, I., Voegele, R. T., Six, J., & Vogelgsang, S. (2019). Use of botanicals to suppress different stages of the life cycle of Fusarium graminearum. Phytopathology, 109(12), 2116-2123. https://doi.org/10.1094/PHYTO-06-19-0205-R
Drakopoulos, D., Meca, G., Torrijos, R., Marty, A., Kägi, A., Jenny, E., Forrer, H.-R., Six, J., & Vogelgsang, S. (2020). Control of Fusarium graminearum in wheat with mustard-based botanicals: From in vitro to in planta. Frontiers in microbiology, 11, 1595. https://doi.org/10.3389/fmicb.2020.01595
Elgat, W. A. A. A., Kordy, A. M., Böhm, M., Černý, R., Abdel-Megeed, A., & Salem, M. Z. M. (2020). Eucalyptus camaldulensis, Citrus aurantium, and Citrus sinensis essential oils as antifungal activity against Aspergillus flavus, Aspergillus niger, Aspergillus terreus, and Fusarium culmorum. Processes, 8(8), 1003. https://doi.org/10.3390/pr8081003
Gwad, M. M. A., El-Sayed, A. S.A., Abdel-Fattah, G. M., Abdelmoteleb, M., & Abdel-Fattah, G. G. (2024). Potential fungicidal and antiaflatoxigenic effects of cinnamon essential oils on Aspergillus flavus inhabiting the stored wheat grains. bmc Plant Biology, 24(1), 394. https://doi.org/10.1186/s12870-024-05065-w
Habschied, K., Krstanović, V., Zdunić, Z., Babić, J., Mastanjević, K., & Šarić, G. K. (2021). Mycotoxins biocontrol methods for healthier crops and stored products. Journal of Fungi, 7(5), 348. https://doi.org/10.3390/jof7050348
Hernández, A., Ruiz-Moyano, S., Galván, A. I., Merchán, A. V., Pérez Nevado, F., Aranda, E., Serradilla, M. J., de Guía Córdoba, M., & Martín, A. (2021). Anti-fungal activity of phenolic sweet orange peel extract for controlling fungi responsible for post-harvest fruit decay. Fungal Biology, 125(2), 143-152. https://doi.org/10.1016/j.funbio.2020.05.005
Holguín-Peña, R. J., Medina-Hernández, D., Vázquez-Islas, G., Nieto-Navarro, F., & Rueda Puente, E. O. (2021). Anti-infective properties of medicinal plants from the Baja California peninsula, Mexico for the treatment of Fusarium oxysporum f. sp. basilici in organic sweet basil (Ocimum basilicum). Revista de la Facultad de Ciencias Agrarias UNCuyo, 53(1), 234-244. https://doi.org/10.48162/rev.39.022
Kochiieru, Y., Mankevičienė, A., Cesevičienė, J., Semaškienė, R., Ramanauskienė, J., Gorash, A., Janavičiene, S., & Venslovas, E. (2021). The impact of harvesting time on Fusarium mycotoxins in spring wheat grain and their interaction with grain quality. Agronomy, 11(4), 642. https://doi.org/10.3390/agronomy11040642
Kulazhanov, T., Uazhanova, R., Baybolova, L., Yerzhigitov, Y., Kemerbekova, A., Tyutebayeva, K., Izembayeva, A., & Zhengiskyzy, S. (2024). Ensuring quality and safety in the production and storage of grain crops. Caspian Journal of Environmental Sciences, 22(5), 1279-1284. https://doi.org/10.22124/cjes.2024.8343
Kursa, W., Jamiołkowska, A., Wyrostek, J. & Kowalski, R. (2022). Antifungal effect of plant extracts on the growth of the cereal pathogen Fusarium spp. - An in vitro study. Agronomy, 12(12), 3204. https://doi.org/10.3390/agronomy12123204
Leslie, J. F., & Summerell, B. A. (2006). The Fusarium laboratory manual. Blackwell Publishing.
Maidana Ojeda, M., Acosta Ramos, M., Cabrera, M. G., Quintana Viedma, L., Enciso Maldonado, G., & Mongelós Franco, Y. (2022). Evaluación in vitro de inhibidores contra hongos micotoxigénicos del maíz. Ciencia Latina Revista Científica Multidisciplinar, 6(2), 3289-3302. https://doi.org/10.37811/cl_rcm.v6i2.2088
Mangoba, M. A. A., & Alvindia, D. de G. (2025). Promising antifungal effects of Citrus maxima peel for the control of banana fruit rot. European Journal of Plant Pathology, 714, 367-376. https://doi.org/10.1007/s10658-025-03135-9
Martinko, K., & Mioč, E. (2024). Antifungal effect of cinnamon bark extract on the phytopathogenic fungus Fusarium sporotrichioides. Food Technology and Biotechnology, 62(4), 458-464. https://doi.org/10.17113/ftb.62.04.24.8448
Mesterhazy, A. (2024). What Is Fusarium head blight (FHB) resistance and what are its food safety risks in wheat? Problems and solutions - A review. Toxins, 16(1), 31. https://doi.org/10.3390/toxins16010031
Morales-Ubaldo, A. L., Rivero-Perez, N., Valladares-Carranza, B., Madariaga-Navarrete, A., Higuera-Piedrahita, R. I., Delgadillo-Ruiz, Bañuelos-Valenzuela, R., L. & Zaragoza-Bastida, A. (2022). Phytochemical compounds and pharmacological properties of Larrea tridentata. Molecules, 27(17), 5393. https://doi.org/10.3390/molecules27175393
Mshari, A., Alrudainy, A. M., & Abu-Mejdad, N. M. J. A. (2022). Study the effect of some citrus peel extracts against plant pathogenic fungi. Asian Journal of Water, Environment and Pollution, 19(6), 103-110. https://doi.org/10.3233/AJW220094
Preciado-Ortiz, R. E., & Vázquez-Carrillo, M., G. (2022). Generación de maíces especializados para mejorar la salud y nutrición en México. aci Avances en Ciencias e Ingenierías, 14(1), 2489. https://doi.org/10.18272/aci.v14i1.2489
Pizzo, F., Caloni, F., Schreiber, N. B., Cortinovis, C., & Spicer, L. J. (2016). In vitro effects of deoxynivalenol and zearalenone major metabolites alone and combined, on cell proliferation, steroid production and gene expresión in bovine small-follicle granulosa cells. Toxicon, 109, 70-83. https://doi.org/10.1016/j.toxicon.2015.11.018
Ramírez-Mejía, J. M., Aguilera-Galvez, C., Kema, G. H. J., Valencia-Riascos, L. M., Zapata-Henao, S., Gómez, L. A., & Villegas-Escobar, V. (2024). Combining cyclic lipopeptides and cinnamon extract enhance antifungal activity against Fusarium oxysporum strains pathogenic to banana and delay Fusarium wilt under greenhouse conditions. Tropical Plant Pathology, 49, 838-849. https://doi.org/10.1007/s40858-024-00677-x
Rodríguez-Guadarrama, A. H., Guevara-González, R. G., de Jesús, R. G. S., & Feregrino-Pérez, A. A. (2018). Antifungal activity of Mexican endemic plants on agricultural phytopathogens: a review. xiv International Engineering Congress. Querétaro, México.
Serrano-Coll, H. A., & Cardona-Castro., N. (2015). Micotoxicosis y micotoxinas: generalidades y aspectos básicos. ces Medicina, 29(1), 143-151.
Sistema de Información Agroalimentario y Pesquero. (2024). Panorama agroalimentario. La ruta de la transformación agroalimentaria (2018-2024). siap. https://online.pubhtml5.com/rsarc/ywrn/
Torrijos, R., Nazareth, T. de M., Vila-Donat, P., Mañes, J., & Meca, G. (2022). Use of mustard extracts fermented by lactic acid bacteria to mitigate the production of fumonisin B1 and B2 by Fusarium verticillioides in corn Ears. Toxins, 14(2), 80. https://doi.org/10.3390/toxins14020080
United States Department of Agriculture. (s. f.). Production - Grains Summary. https://www.fas.usda.gov/data/production/commodity-group/grains
Viljoen, J. J. F., Truter, M., & Kritzinger, Q. (2025). Mycoflora and mycotoxins associated with stored cowpea (Vigna unguiculata L. Walp) seeds from smallholder farmers in South Africa. Phytoparasitica, 53, 44. https://doi.org/10.1007/s12600-025-01267-6
Wigmann, É. F., Behr, J., Vogel, R. F., & Niessen, L. (2019). maldi-tof ms fingerprinting for identification and differentiation of species within the Fusarium fujikuroi species complex. Applied Microbiology and Biotechnology, 103, 5323-5337. https://doi.org/10.1007/s00253-019-09794-z
Zhou, L.-R., Hu, H.-J., Wang, J., Zhu, Y.-X., Zhu, X.-D., Ma, J.-W., & Liu, Y.-Q. (2024). Cinnamaldehyde acts as a fungistat by disrupting the integrity of Fusarium oxysporum Fox-1 cell membranes. Horticulturae, 10(1), 48. https://doi.org/10.3390/horticulturae10010048
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